GSM7062246: LMNB1, PML knockdown, MDA231, rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
LMNB1
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-231
cell line
MDA-MB-231
cell type
Human breast cancer
chip antibody
anti-LMNB1 Abcam 16048
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7062246
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 2 mM Di(N-succinimidyl) glutarate for 45 min at RT and with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using the Diagenode Bioruptor 300. ChIP-seq libraries were prepared using ChIPSeq Illumina protocol. Libraries were validated using the High Sensitivity D5000 ScreenTape (Agilent Technologies).